Plasmid_Backbone
LT

Part:BBa_K2144101:Design

Designed by: Sigrun Stulz   Group: iGEM16_Stockholm   (2016-10-14)


Vector containing Hisx6 and Sortase tag LPETGG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2029
    Illegal SpeI site found at 2099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2029
    Illegal SpeI site found at 2099
    Illegal NotI site found at 2106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2029
    Illegal BamHI site found at 2038
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2029
    Illegal SpeI site found at 2099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2029
    Illegal SpeI site found at 2099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

BamHI is a very convenient restriction enzyme for our application as its restriction site will code for a glycine and a serine, both of which are often used in linkers and tend to be unproblematic in such sequences.


Source

The plasmid was constructed using the iGEM-supplied pSB1C3 and a synthetically produced sequence.

References